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An outer strip showing coloured filaments of uncertain origin was discarded.

The remaining sample, measuring 81 mm × 16 mm (3.19 in × 0.63 in) and weighing 300 mg, was first divided in two equal parts, one of which was preserved in a sealed container, in the custody of the Vatican, in case of future need.

Since the C14 dating at least four articles have been published in scholarly sources contending that the samples used for the dating test may not have been representative of the whole shroud.

These included an article by American chemist Raymond Rogers, who conducted chemical analysis for the Shroud of Turin Research Project and who was involved in work with the Shroud since the STURP project began in 1978.

Gove consulted numerous laboratories which were able at the time (1982) to carbon-date small fabric samples. [...] The pressure on the ecclesiastic authorities to accept the Turin protocol have almost approached illegality.

group published the list of tests to be performed on the shroud; these aimed to identify how the image was impressed onto the cloth, to verify the relic's purported origin, and to identify better-suited conservation methods. We are faced with actual blackmail: unless we accept the conditions imposed by the laboratories, they will start a marketing campaign of accusations against the Church, which they will portray as scared of the truth and enemy of science.

He stated that his analysis showed: "The radiocarbon sample contains both a gum/dye/mordant coating and cotton fibers.

The main part of the shroud does not contain these materials." He speculated that these products may have been used by medieval weavers to match the colour of the original weave when performing repairs and backing the shroud for additional protection.

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It is hypothesised that the sampled area was a medieval repair which was conducted by "invisible reweaving".Luigi Gonella claimed to have taken from the radiocarbon sample before it was distributed for dating.The actual provenance of these threads is uncertain, as Gonella was not authorized to take or retain genuine shroud material, Raymond Rogers stated in a 2005 article that he performed chemical analyses on these undocumented threads, and compared them to the undocumented Raes threads as well as the samples he had kept from his STURP work. group and the candidate laboratories turned into a P. However, in a 1990 paper Gove conceded that the "arguments often raised, … that discarding the blind-test method would expose the results – whatever they may be – to suspicion of unreliability.The other half was cut into three segments, and packaged for the labs in a separate room by Dr Tite and the archbishop.